Binding of an aptamer to the N-terminal fragment of VCAM-1

Abstract In vitro selection of 2′-fluoropyrimidine oligonucleotide aptamers was performed against the N-terminal two-domain fragment of mouse VCAM-1. The SELEX procedure enriched the starting pool in a family of homologous sequences. High binding affinity (10 nM) of one member of this family, aptamer 12.11, was demonstrated in a filter binding assay.