Fermentative Production of cis-4-Hydroxy-(L)-proline by Helicocerus oryzae and Acrocylindrium oryzae

The production of hydroxyproline by microorganisms was studied to discover the chiral source of the side chain moiety of carbapenem. cis-4-Hydroxy-(l)-proline produced by Helicocerus oryzae and Acrocylindrium oryzae was purified from culture broths. The 1NMR spectra and [α]D of purified samples were in agreement with those of standard samples. Our finding provided the first evidence for fermentative production of cis-4-hydroxy-(l)-proline. The addition of (l)-proline to the culture broth of H. oryzae increased the production of cis-4-hydroxy-(l)-proline.