Effects of hyperglycemia and advanced glycation end-products on osteogenesis ability of human adipose-derived stromal cells

Objective To explore the effects of hyperglycemia and advanced glycation end-productes (AGEs) on osteogenesis of human adipose-derived stromal cells (ADSCs). Methods Human ADSCs were isolated and induced to the bone formation. 27.5 mmol/L high glucose concentration and 100 mg/L AGEs were used to simulate the diabetic environment. There were four groups in the present study, 6 specimens in each group. Through immunofluorescence detection and observation of calcification in each group,the different changes in CNI secretion and osteogeniesis ability were statistically analyzed. Results Hyperglycemia and AGEs prevented ADSCs cognate differentiation into bone. On the 21st day, the CNI amount in combination group was 2.76 times lower than the normal group, and the average calcification nodes were (4.10 ± 1.24). There was significant difference between two groups (P ＜0.01 ). Conclusion Hyperglycemia and AGEs had a deleterious effect on osteogenesis ability of ADSCs. Key words: Hyperglycemia;  Bone;  Differentiation;  Adipose-derived stromal cells