Altered phospholipid metabolism in a temperature-sensitive mutant of Escherichia coli, CR34T46

1972 
Abstract The phospholipid metabolism of a temperature-sensitive DNA initiation mutant of Escherichia coli K12, CR 34 T 4 6, is altered at the restrictive temperature, 40 °C. The relative rate of synthesis and the relative proportion of the anionic phospholipids (phosphatidylglycerol and cardiolipin) were increased in the mutant when it was grown at 40 °C. Glycerol-3-phosphate : CMP phosphatidyltransferase, the enzyme catalyzing the first step in the synthesis of anionic phospholipids, showed increased activity in membrane preparations of the mutant grown at 40 °C ; this finding could explain the altered phospholipid composition. Phospholipid metabolism was similar in CR 34 T 4 6 and revertants capable of growth at 40 ° C. Thus, the altered phospholipid metabolism appears to be independent of the DNA initiation lesion.
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