Detection of genetically modified organisms in food

PCR methods for the detection of genetically modified organisms (GMOs) were developed that can be used for screening purposes and for specific detection of glyphosate-tolerant soybean and insect-resistant maize in food. Protocols for amplification of parts of the 35S promoter derived from Cauliflower Mosaic Virus, the NOS terminator derived from Agrobacterium tumefaciens and the antibiotic marker gene NPTII (neomycin-phosphotransferase II) allow for general screening of foods. PCR/hybridisation protocols were established for the detection of glyphosate-tolerant RoundUp Ready soybean and insect-resistant Bt-maize. Besides hybridisation, confirmation of results using restriction analysis was also possible. Highly sensitive detection of GMOs - in most cases 0.01% - could be achieved.