Method for measuring effective ingredients in Huoxiang Zhengqi oral liquid

The invention provides a method for simultaneously measuring the contents of three ingredients including hesperidin, magnolol and honokiol in a Huoxiang Zhengqi oral liquid by use of a two-stage gradient elution method. The method is suitable for a high performance liquid chromatography (HPLC), an ultra performance liquid chromatograph (UPLC) and other liquid chromatographs, and is also suitable for chromatographic columns, taking octadecylsilane chemically bonded silica (C18) as a filler, of various models and specifications. Meanwhile, the method is suitable for simultaneously measuring the three ingredients including hesperidin, magnolol and honokiol in the Huoxiang Zhengqi oral liquid, that is, the method is suitable for quality control of the Huoxiang Zhengqi oral liquid and a Huoxiang Zhengqi liquid. The method has good universality on various liquid chromatographs and the chromatographic columns taking C18 as the filler, and a sample preparation method is simple, high in stability, simple and convenient to operate, safe, quick, accurate and reliable and high in efficiency.