A Genome-Wide Identification of Target Genes Responsible for Anti-Adipogenic Activity of D-Psicose, a Sugar Substitute, in C57BL/6J-ob/ob Mice

Excess intake of added sugar has been shown to increase visceral fat and the risk of metabolic diseases including obesity and type II diabetes. In an attempt to decrease sugar intake, rare sugars and their derivatives that exist in limited quantities in nature, have been recently developed as a sugar substitute. D-psicose, a C-3 epimer of D-fructose, is known to have anti-adipogenic property. However, the mechanistic explanation on its anti-adipogenic effect has not been clearly understood. Here, we investigated the effect of dietary D-psicose substitution for sucrose on adipose tissue fat deposition and identified target genes. C57BL/6J ob/ob mice were assigned to receive either the AIN-93G or the 5% D-psicose diet for 12 weeks. At necropsy, blood and white adipose tissue samples were collected for further analyses. We performed genome-wide analysis of gene expression in white adipose tissue using Illumina MouseRef-8 and Ingenuity Pathway Analysis was used for network analysis. The mRNA expression of gen...