Влияние нарушений обмена железа организма на течение экспериментальной оппортунистической инфекции

The investigation results of the influence of iron metabolism disturbances on the translocation and survival of P. aeruginosa and S. aureus have been presented. Modeling iron deficiency anemia (IDA) and hemosiderosis (HS) was carried out on laboratory mice by introducing desferrioxamine and iron sulphate (III), respectively. Infection was carried out intraperitoneally (dose 1/2LD 50 per mouse) and animals were withdrawn from the experiment 12 h - 48 h - 72 h - 15 days after being infected. We determined the degree of microbial contamination CFU/g of the internal organs (liver, spleen), the genes expression of hemolytic phospholipase C ( plcH ), alginate ( algD ), exotoxin A ( exoA ) for P. aeruginosa and protein A gene ( spaA ), a global regulator of virulence ( argABCD ) for S. aureus . The development of sepsis and lethal outcomes may be observed in HS. IDA reduces the expression of virulence factors of P. aeruginosa , but contributes to the persistence of the parasite. By contrast, the sepsis development and the increase in the expression of general genes of pathogenicity occur for S. aureus in IDA. Different effects of iron metabolism disturbances on the course of experimental opportunistic infection are being discussed from the position of «quorum sensing» system in bacteria.