Effect of 2-[(3-carboxy-1-oxoprogyl) amino]-2-deoxy-D-glucose on esophageal cancer in nude mice and expression of its relational genes

Objective To study the influence 2-[(3-carboxy-1-oxoprogyl) amino]-2-deoxy-D-glucose glucose(COPADG) on esophageal carcinoma nude mice,and the expression of related genes in tumors.Methods The models of esophageal carcinoma in nude mice were established.Experimental group was divided randomly into control group,COPADG group,5-FU group and combination group(CF).The rate of suppression of tumor was calculated and the expression of PCNA,caspase3,VEGF and MVD were detected by immunohistochemistry.The apoptosis of the tumors was detected by flow cytometry.Results The rate of suppression of tumor COPADG,5-FU,CF groups were 54.16%,38.78%,73.91% respectively.The expression of VEGF,MVD in COPADG,5-FU,CF groups were lower than control group(P0.01).The expression of caspase3 in COPADG,5-FU,CF groups were higher than control group(P0.01).The expression of PCNA in COPADG,CF groups were lower than control and 5-FU groups(P0.01).The apoptotic cell rates in the early stage in COPADG,5-FU,CF groups were higher than control group(P0.01).Conclusion COPADG can inhibit the growth of the human esophageal carcinoma in nude mice by suppressing proliferation,angiogenesis and induced apoptosis of the tumor.