A kind of bacillus subtilis inductivity strong promoter and its application

The invention proposes a kind of promoters, have nucleotide sequence shown in SEQ ID NO:1.Promoter proposed by the present invention is bacillus subtilis inducible strong promoter, can be used for constructing the recombinant bacterial strain of high expression beta galactosidase and high yield Surfactin.The activity of recombinant bacterial strain expression beta galactosidase is 3.6~5.5 times containing promoter Pg2 (nucleotide sequence shown in SEQ ID NO:4) recombinant bacterial strain.The recombinant bacterial strain Surfactin shaking flask yield is up to 9.74g/L, is 17.7 times of wild strain under same culture conditions.Bacillus subtilis inducible strong promoter of the present invention can also be commonly used to the high bacterial strain for expressing other foreign proteins and building high yield Lipopeptide Biosurfactants.