Variation mechanism of HMW-GS in progenies of wheat distant hybridization.

The compositions of high-molecular-weight glutein subunits(HMW-GS) of F1 grains derived from wheat Chuannong19(N,7+8,2+12)×Chuannong18(1,7+9,2+12) were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE),and a HMW-GS variant was detected in one of the grains tested.The HMW-GS patterns of the variant seed were(1,x+7+8+9,2+12).SDS-PAGE analysis showed that the band of variant subunit was similar to 1Bx6,which located between 1Dx5 and 1Bx7.The HMW-GS subunits encoded by Glu-B1 and the combination of Glu-A1 and Glu-B1 loci deviated the Mendel's segregation ratios in F2 progenies.Furthermore,the variant subunit and the 1By8 were codominant.The forming mechanism of the variant HMW-GS was discussed at last.