[Cloning, characterization and application of the promoter region of the alkaline protease gene in Bacillus alcalophillus PB92].

Promoter function fragment of alkaline protease gene(GenBank accession number:EU130686) was cloned from Bacillus alcalophillus PB92 genome by TAIL-PCR.Sequenced and analyzed revealed that it contains several typical promoter characterized regions.Two reverse translation frames were located in-538～-370 bp and -275～-128 bp.Deletion analysis of the sequence demonstrated that 414 bp to 619 bp upstream of the TSS showed predominant promoter activity, and a 105 bp length sequence can serve as this function.Additionally,a representative Sec-type signal peptide structure was detected in PB92 AprE signal peptide.By cloning the PaprE and alkaline protease signal peptide gene into pBE2,an expression vector pBEAC was constructed,and a plant sweet protein monellin gene was highly expressed in B.subtilis 1A751.