The effect of 30-day pretreatment with pentoxifylline on the survival of a random skin flap in the rat: An ultrastructural and biomechanical evaluation.

The aim of this study was to clarify the histological, ultrastructural and biomechanical effects of pentoxifylline (PTX) on the survival of random skin flaps (RSFs) in rats. Thirty male rats were randomly divided into experimental, sham and control groups. The experimental group received PTX 20 mg/kg/day, and the sham group received saline. A 20×70-mm RSF was made 30 days after the commencement of treatment for the three groups. PTX and saline were continued postoperatively for 7 days in the experimental and sham groups, respectively. On the seventh postoperative (lay, the surviving parts of the flaps were determined and examined through light and transmission electron microscopes. The wounds (incisions) on the margins of the flaps were evaluated histologically and biomechanically. Analysis of variance showed that, in the experimental group, the mean of the surviving parts of the RSFs, fibroblast proliferation, collagen organization and granulation tissue of the wounds was significantly higher than in the sham and control groups (P=0.007, P=0.001, P=0.041, P=0.000, respectively). There were swollen mitochondria in the endothelium of tlie blood vessels of the surviving flap parts in the control and sham groups, whereas in the experimental group the mitochondria were normal. Thirty days of pretreatment of RSFs with PTX significantly increased the survival of the flaps. PTX appealed to have healed wounds and reversed ultrastmetural changes resulting from hypoxia in the blood vessel endothelium of the flaps.