In Silico Analysis of HA2/Mx Chimera Peptide for Developing an Adjuvanted Vaccine to Induce Immune Responses Against Influenza Viruses
2015
Purpose: The direct transmission of avian influenza viruses to human and
increasing drug resisted strains posing new threats for public health. Therefore,
development of efficient vaccines is needed
to generate protective and persistent immunity to the viruses.
Methods: Three motifs of Mx protein sequence in human, mouse and poultry located in interferon induced (GTP ase) domain were
candidate as biologic adjuvant for enhancing the immune responses against
influenza virus. Chimera proteins composed with the conserved HA2 subunit of influenza virus and the Mx
motifs named HA2/Mx were modeled and evaluated by in silico analysis includes bioinformatics algorithms in
order to explore biological characteristics of these peptides.
Results: Amongst the predicted models, HA2/Mx1 peptide showed the
better results following protein structures prediction, antigenic epitopes
determination and model quality evaluation. Comparative homology modeling was
performed with Swiss Model and the model was validated using ProSA. Epitope predictions
revealed the construct could induce both B and T cell epitopes that expect a
high immune response.
Conclusion: Taken together, these data indicate that the HA2/Mx1 chimera
peptide can be potentiated for developing an adjuvant-fused influenza
vaccine capable of stimulating effective immune response.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
41
References
4
Citations
NaN
KQI