Studies on Initial Adsorption Process of Hemoglobin onto the Glass Surfaces by Using Langmuir Adsorption Isotherm

The adsorption process of hemoglobin onto glass surfaces having different hydrophobicities was studied using slab optical waveguide (SOWG) spectroscopy. Non-treated glass was employed as a hydrophilic surface. Hydrophobic glass surfaces were treated by octyltrichlorosilane (OTS) in toluene. Two distinct stages of adsorption process were observed with hydrophobic surface. The rapid attainment of the steady-state (up to 2 minutes) of the absorbance value was also noticeable when using a hydrophilic surface. The adsorption data obtained for hemoglobin on both hydrophilic and hydrophobic surfaces were examined using the Langmuir’s adsorption isotherm at extremely short time (up to 12 seconds). Results showed that the first stage of adsorption process followed the Langmuir’s adsorption isotherm.