Recombinant proteasome alpha-type subunits exhibit endoribonuclease activity

26S proteasome is a multisubunit protein complex that consists of 19S regulatory and 20S catalytic subcomplexes. The primary proteasome cellular function is protein degradation. It has recently been found that, in addition to its proteolytic activities, the 20S particle also displays endoribonuclease activity mediated by two alpha-type subunits, α1 and α5. In this report, we have analyzed other alpha-type subunits for their ability to hydrolyze RNA. We have found that all of the recombinant subunits tested (α1, α2, α3, α4, α5, α7) exhibited endoribonuclease activity that depends on the origin of RNA and the presence of bivalent ions in the reaction. These results indicate that the endoribonuclease activity of proteasomes may play an important role in cellular RNA metabolism.