Method for enzymatic synthesis of glucose ester of fatty acids in organic phase

The invention relates to a synthesis method of a glucose fatty acid ester by the enzyme catalysis in an organic phase, belonging to the food biochemical technical field, which is characterized in that a glucose lauric acid ester, a glucose myristate, a glucose ascorbyl palmitate and a glucose stearate are respectively catalyzed and synthesized in an acetone with a fatty enzyme NOVO435, identifying the optimized production process conditions and the product separation and purification method. The transformation of the glucose lauric acid ester is up to 80.2%, the purity of the monoester is over 99% after separation and purification under the optimized process conditions. The synthesis method of the glucose fatty acid ester by the enzyme catalysis in an organic phase has the advantages of moderate conditions, high transformation rate, easy separation and purification, and green security.