Identification of diagnosis markers for pulmonary tuberculosis by proteomic fingerprinting.

The objective was to study the diagnosis method of active pulmonary tuberculosis using protein fingerprinting technology.A sample of 130 sputum-bacteriologically positive and negative patients with active pulmonary tuberculosis and 65 healthy individuals as control were selected from clinically diagnosed cases of Mycobacterium tuberculosis in the hospital to conducted protein fingerprinting test,and the associated protein peak values were then examined using statistic analysis.Results showed that five protein peaks(5335,8048,11683,11700,and 11526m/z)increased significantly in all 130 active pulmonary tuberculosis patients compared to the 65 control individuals(P0.01).This diagnostic model showed a diagnostic accuracy of 92.3%(180/195),specificity of 100%(65/65),and sensitivity of 88.5%(115/130)respectively.In the 65 sputum-bacteriological positive cases,24 with protein peak at 5335 m/z,16 at 11683 m/z,14 at 8048 m/z,and 5 at 11700 m/z;in the 65 sputum-bacteriological negative cases,20 with protein peak at 8048 m/z,14 at 5335 m/z,8 at 11526 m/z,7 at 11683 m/z,and 6 at 11700 m/z.It's indicated that serum protein fingerprinting technology is fast,simple,and consumes a relatively small amount of samples.As a result,it is an effective method for identifying the unique markers of pulmonary tuberculosis and could become a standard in early diagnose.