Proteomic analysis of serum from patients with polycystic ovary syndrome and insulin resistance

Objective: To find out disease specific-proteins(DSPs) which are closely related with polycystic ovary syndrome(PCOS) and insulin resistance(IR).Methods: The serum proteins were separated by immobilized pH gradient-based two-dimensional gel eletrophoresis(2-DE).The gels were stained by silver,scanned by gel formatter,and analyzed by using PDquest software.The differentially displayed protein spots associated with PCOS IR were searched for and identified by MALDI-T0F-MS.Results: Five DSPs were chosen for test and two DSPs were found,which were haptoglobin and RhoA protein.Conclusion:There are different protein expressions in 2D-PAGE of PCOS IR and non-IR PCOS.These proteins may be DSPs of PCOS IR,and may be the markers for diagnosis.