Detection of Carbofuran Pesticide Using an Enzyme Biosensor Based on MWNTs-PDDA Modified Glassy Carbon Electrode

This paper presents the construction of an amperometric biosensor for the highly sensitive detection of the carbamate insecticide carbofuran, based on the inhibition of acetylcholinesterase (AChE). Using an enzyme biosensor based on multi-walled carbon nanotubes (MWNTs) and poly diallyldimethylammonium chloride (PDDA) modified glassy carbon electrode (GCE), then immobilizing the Acetylcholinesterase (AChE) to the modified GCE with layer-by-layer (LBL) technique directly. Detect the inhibition of Aches with the amperometric i-t response. The pesticide concentration is correlated to the percentage inhibition of enzyme activity. Then detect the carbofuran by the enzyme electrode with 0.01U activity value and it shows a good linearity in the range from 10 -12 g/L to 10 -6 g/L between the inhibition rates and -lg[carbofuran], and the detection limit is 10 -12 g/L.