Next-generation sequencing-based bulked segregant analysis for QTL mapping in the heterozygous species Brassica rapa

Key message An improved protocol of QTL-seq, an NGS-based method for bulked segregant analysis we previously developed in rice, allowed successful mapping of QTLs of interest in the highly heterozygous genome of B. rapa, demonstrating the power of this elegant method for genetic analyses in heterozygous species of economic importance.