Fluorescence lifetime imaging endoscopy
2011
We present two FLIM endoscopes for clinical imaging and in vivo cell biology. For subcellular confocal imaging we
demonstrated the first confocal FLIM endomicroscope, implementing TCSPC with a Cellvizio®GI, which we have now
developed as a self-contained wheeled instrument (1.0 × 0.7 m) incorporating a tunable excitation laser and acquiring
images in < 10 s. This has been applied to image FRET in live cells and to image tissue autofluorescence, for which we
are implementing "FIFO" for image montaging. For diagnostic screening/guided biopsy, we have developed a
complementary wide-field FLIM endoscope employing time-gated detection with violet and UV excitation for imaging
over mm-cm fields of view.
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