La recherche d'anticorps irréguliers sur GroupamaticA la lumière de l'expérience des Centres de Transfusion Sanguine de Paris (C.N.T.S.) et de Toulouse (C.R.T.S.)

Summary We report here the experience of 3 years of irregular antibody automated screening with Groupamatic, that is to say of 661,511 samples tested in Paris and 269,162 samples tested in Toulouse. The positive reactions in Paris were 16,296 (2.46 %) out of which 2,021 irregular antibodies were identified (0.30 %). The positive reactions in Toulouse were 8,266 (3.07 %), out of which 2,138 irregular antibodies were identified (0,79 %). The difference between the number of screened positive reactions and the identified one is due to false positive reactions (half of the cases) and to autoantibodies whose number is roughly the same than the number of identified alloantibodies. During the years 1970, 1971 and 1972, the irregular antibodies were systematically screened in Toulouse on all blood donors with a manual technique on opaline plate using enzyme treated red cell tests (papain). 7,147 positive reactions were detected, out of 240,080 tests (2.98 %). 1,954 (0,81 %) were alloantibodies and 1,529 (0.64 %) autoantibodies ; 3.455 were false positive reactions and 209 were non identified antibodies. These figures are superimposed with those obtained with Groupamatic during the following years, thus pointing out the interest of this equipment.