The effects of simulating static magnetic fields of magnetic attachments on the TGF-β1 mRNA of osteoblasts

Objective To explore the effects of static magnetic fields(SMF) of dental magnetic attachment system with different intensities on the expression of TGF-β1 mRNA of rat osteoblasts.Methods The cultured rat osteoblasts were exposed continuously to 12.5 mT,125 mT,and 250 mT SMF for 0.5,1,3,5,and 7 days,respectively.While,controls were also set up free of SMF.Real-time quantitative PCR was employed to detect the gene expression of cellular TGF-β1 mRNA and the statistical analysis was performed.Results Osteoblasts exposed to 12.5 mT SMF for 0.5,1,and 3 days exhibited an increase on cellular TGF-β1 mRNA expression,as compared to those of the control group(P 0.05).Moreover,after 0.5,1,3,5,and 7 days SMF exposure the cellular TGF-β1 mRNA expression in 125 mT and 250 mT groups were significantly higher than those of the control group and the 12.5 mT group(P 0.05).It showed a dose-effect relationship between the magnetic intensity and the expression of cellular TGF-β1 mRNA.Conclusions Under this experimental condition,the static magnetic fields of magnetic attachments can stimulate the expression of cellular TGF-β1 mRNA in rat osteoblasts,as to enchance the differentiation of osteoblasts.