Inhibition of Proliferation of Colorectal Cancer Cells by Phenolic Extracts of Mandarin (Citrus reticulate) and Lime (Citrus aurantifolia) Fruit Waste

Several epidemiological studies have suggested that phenolics have antineoplastic activities. The objective of this study was to determine the antiproliferative effect of rich phenolics extracts of mandarin (Citrus reticulate) and lime (Citrus aurantifolia) fruit waste in colorectal cancer cells, RKO (carcinom) and HT-29 (adenocarcinom). Antiproliferative effect was evaluated using the methylthiazolydiphenyl-tetrazolium bromide (MTT) assay and testing phenolics extracts from different mandarin and lime fruit waste drying temperatures (fresh, 60, 90, 120oC), at different times (24, 48 and 72 h). Mandarin and lime phenolics extracts inhibit cell viability of colon cancer cell lines, HT-29 and RKO, at 24 h to 72 h. Results suggested that phenolics extracts had different action mechanisms on each type of cells and therefore, more studies were required to elucidate such mechanisms.