COMPUTER CONFORMATION ANALYSIS OF TATA-BOX SEQUENCES IN EUKARYOTIC PROMOTERS

We have developed a method for revealing local conformation properties of binding sites for tran- scription factors, based on conformation indices of B-sheet DNA hexanucleotide duplexes and computer sys- tem SITEVIDEO. Analysis of sequences with TATA box has revealed the promoter regions with certain con- formation indices of B-sheet DNA significantly changed as compared with arbitrary sequences: TATA-box DNA has a lower angle of helical twist, smaller distance between neighbor base pairs along the DNA helix axis, wider minor and narrower major DNA grooves.