A Urinary Metabonomics Research on Total Ginsenoside Treated Diabetes Cardiomyopathy Rats Based on Rapid Resolution Liquid Chromatography/Mass Spectrometry

A urinary metabonomics method based on rapid resolution liquid chromatography/mass spectrometry (RRLC/MS) was developed to study the effect mechanism of Total Ginsenoside (TG) on streptozotocin(STZ) induced Diabetic Cardiomyopathy (DCM) rats. Twenty-four STZ-induced diabetic rats were randomly divided into DCM group and TG group. DCM group was administrated with TG 30 mg/kg body weight once a day by gastric irrigation. TG group was administrated with water by gastric irrigation in parallel. Ten healthy rats were defined as control group and were also administrated with water by gastric irrigation in parallel. After twelve-week continuous irrigation, 24 h urine samples of rats in the three group were collected and analyzed by RRLC/MS. Principal component analysis (PCA) was carried out for pattern recognition and a clear separation between DCM rats and those treated with TG was achieved. Three potential biomarkers were found and identified. The significant changes of these biomarkers indicated the pharmacological effects of TG on the metabolism of rats. TG can regulate the metabolism of body include citrate cycle, fatty acid metabolism and oxidative stress. The results shows the metabonomics is a useful tool in effect mechanism research of natural product and this work is helpful in the effect mechanism study of Panax ginseng.