Gene detection of airway inflammation relevant biomarkers

Objective To search clinical biomarkers of the progression of allergic rhinitis toward asthma.Methods Inferior turbinate mucosa was obtained from 6 SAR patients and 5 SAR patients with asthma,respectively.Total RNA was extracted from the nasal mucosal biopsies,and biotin-labeled cRNA probes were hybridized with Affymetrix Human Genome HG-U133-plus2.0 Array.The hybridization results were confirmed by RT-PCR analysis.A comprehensive data and literature mining was performed to differential expression genes using Pubmed-NLM and GeneCard database.Results In GeneCard database,354 genes were related with asthma,45 genes were related with allergic rhinitis,and 13 genes were related with allergic asthma.Compared with SAR patients,SCCA1(SERPINB3)and SCCA2(SERPINB4)were highly expressed in nasal mucosa of SAR patients with asthma at mRNA level.SCCA1 and SCCA2 were further identified as two important signs of the progression of allergic rhinitis toward asthma by integrating array data with array-independent literature mining.Conclusion Expression level of SCCA1(SERPINB3),SCCA2(SERPINB4)genes in nasal mucosa could be relevant clinical biomarkers of the progression of allergic rhinitis toward asthma.