The IgE-diagnostic impact of a recombinant serine protease inhibitor from Hevea brasiliensis in latex-allergic health care workers

» Amplifi cation of the SPI-sequence from a cDNA of Hevea brasiliensis » Identifi cation of the sequence in a pDrive vector system (EMBL accession number: HF937118) » Expression of the SPI variant in the pMAL system » Preparative isolation of a soluble, recombinant maltose-binding protein (MBP)-SPI fusion protein » Biotinylation of the SPI-hybrid and coupling onto StreptavidineImmunoCAPs (ThermoFisher Scientifi c, Uppsala , Sweden) » 21 sera of health care workers (HCW) with symptoms to natural rubber latex (NRL) were tested for SPI and all available single latex allergens