A simple method for detecting and quantifying microplastics utilizing fluorescent dyes - Safranine T, fluorescein isophosphate, Nile red based on thermal expansion and contraction property

Microplastics (particle size <5mm) are an emerging contaminant for aquatic environmental, which have attracted increasing attention in worldwide range. In this study, an improved fluorescent staining method for detection and quantification of microplastics was developed based on thermal expansion and contraction. This method is effective in detection of polyethylene, polystyrene, polyvinyl chloride and polyethylene terephthalate plastic particles. In order to avoid error statistics caused by pretreatment, various characterizations of microplastics were measured after heated, such as microstructure, compositions and thermostability. The results showed that there was no significant damage to microplastics even under heating condition at 75 degrees C for 30min, and the stained microplastics had strong stability for up to two months. Moreover, this method has been successfully applied to the quantification of microplastics in biological samples and result showed there were about 54 particles g(-1) (dry weight) microplastics in the Sipunculus nudus. This new method provides a reliable method for quantitative analysis of microplastics in environment and biological tissue.