Characterization of the Insulin and Transferrin Receptors of Two Cancer Cell Lines.

The insulin and transferrin receptors of the PA-1 and GR2H6 cell lines were characterized respectively.The optimum pH for the binding of PA-1 cell is 7.0with insulin and with transferrin is 7.5～8.0.Scatchard plot and Hill plot analyses show respectively the receptor number of 2.97×106/cell and 6.21×107/cell for insulin receptors of PA-1 and GR2H6 with kD of 4.16×10-7M and 7.57×10-7M,Rill coefficient of 0.78 and 0.82,Showing a negative cooperativity;transferrin receptors of PA-1 and GR2H6 were found to have respectively receptor number of 1.25×105/cell and 1.40×105/cell.with kD of 1.38×10-9M and 1.66×10-8M.The Hill coefficient was 1.04 and 0.98,almond no different from 1,so transferrin receptors showed no cooperativity.