Morphofunctional condition of the harderian gland of chickens during the post-vaccine immunity to infectious bronchitis
2020
Aim. To fi nd out the contents and placement of CD4+, CD8+, CD45RA+, CD20+-lymphocyte subpopulations
and morphocytochemical architecture of the harderian gland in clinically healthy chickens and for vaccination
against infectious bronchitis. Methods. Anatomical, histological, immunohistochemical, optical, morphometric
and variational-statistical. Results. The obtained morphometric study results showed that the area of secretory
lobules and lymphoid formations was signifi cantly larger in the harderian gland of vaccinated 40-, 90- and
110-day-old chickens. Moreover, the organ size correlates signifi cantly with the development of lymphoid
formations, since the area of the secretory lobules in the gland of the experimental group chickens does not differ
from that of the control group. Immunohistochemical studies have shown that the number of lymphocytes
with CD20+ markers was characterized by a reliable (P < 0.001) increase in B-lymphocytes in the harderian
gland of the vaccinated chickens of all ages. Lymphocytes with CD8+ markers were not detected in the gland
of vaccinated 8- and 20-day-old chickens. According to cytomorphometric studies, their number in vaccinated
40-, 90- and 110-day-old chickens was signifi cantly (P < 0.001) higher compared to intact birds – 2.11, 2.38
and 2.96 times, respectively. Conclusions. The data on the composition of lymphocytes with CD4+, CD8+,
CD45RA+, CD20+ markers in the harderian gland of chickens of different age, on their changes in case of immunization
against infectious bronchitis were obtained, and the role of certain clusters at different stages of the
immunity formation was determined. The information on the structure, patterns of growth and development
of the harderian gland of chickens, which demonstrates the incompleteness of its morphogenesis at the early
stages of the postnatal ontogenesis period that should be taken into account during the compilation of vaccine
prophylaxis programs, has been supplemented.
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