Methods housekeeping protein tissue protein detection

2012 
The present invention provides a method of using tissue housekeeping proteins rapid detection of proteins, the detection method housekeeping proteins, the protein was detected with monoclonal or polyclonal, respectively conjugated colloidal gold or colloidal silver, formed after the coupling colloidal silver or colloidal gold-antibody complex was added dropwise to a gold standard test strip pad; in another test strip on the respective anti housekeeping proteins or monoclonal or polyclonal protein detected in a test strip fixed to the detection zone Finally, housekeeping proteins, the relative amount of color in the color according to the ratio of test proteins is calculated on the test strip, and then determine the expression level of the protein to be detected is negative or positive. The detection method solves the colloidal gold immunoassay method for quantitative problems, expanding its application in the detection of protein; Further, the detection method with respect to other protein detection technology speed, high accuracy and other advantages.
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