Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study.

This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy-Delta12,14-PG J2 (15d-PGJ2), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGFbeta-1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated with LPS 10 mug/ml and treated with 15d-PGJ2 1 and 2 mug/ml, and ScLL 2 and 5 mug/ml, for 1 and 3h, and then evaluated for viability by MTT assay. Supernatant was collected to detect IL-6 and TGFbeta-1 release, by ELISA. Positive control was cells kept in Dulbecco's Modified Eagle's Medium, and negative control was those kept in LPS. Data were analyzed by ANOVA and Dunnett's test (alpha = 0.05). No significant difference was found in viability among experimental groups at 1h (p > 0.05). Percentage of ScLL 5 microg/ml viable cells was similar to that of positive control at evaluated periods (p > 0.05), whereas the other groups had lower levels than the positive control (p 0.05), except for ScLL 2 microg/ml and 15d-PGJ2 1 microg/ml, which showed lower IL-6 release compared with that of negative control (p 0.05). Results indicated that ScLL 5 mug/ml did not interfere in viability, and ScLL 2 microg/ml and 15d-PGJ2 1 microg/ml demonstrated reduced IL-6 release. Tested substances had no effect on TGFbeta-1 release.