Targeted anti-ICAM-1 microbubbles mediated hAng-1 gene transfection in treatment of ischemic myocardium in rabbits

Objective To investigate whether the anti-ICAM-1 targeted microbubble can improve the transfection efficiency of hAng-1 gene in myocardial infarction.Methods The combination degree of anti-ICAM-1 targeted microbubbles with human interleukin-1βstimulated ECV304 cells was detected in vitro. And for animal study,myocardial infarction rabbits were divided into three groups:control group,non-targeted microbubbles delivery group and targeted microbubble delivery group.The hAng-1 gene was transferred into vein by three methods under ultrasound irradiation.The condition of infarcted myocardial perfusion was detected by cardiomyography after two weeks,and mRNA expression of hAng-1 gene was detected by RT-PCR,which was used to assess gene transfection efficacy.Results In vitro,a large number of anti-ICAM-1 microbubbles could targeted combine with ECV304 cells which responsed to inflammatory reaction.After two weeks of gene transfection,the diameter of left ventricular was decreased,and LVEF was increased,while there was no significant difference between targeted microbubble delivery group and non-targeted microbubbles delivery group (P0.05).And cardiomyography showed that contrast agent filling in myocardium was more than that in non-targeted microbubbles delivery group,and the hAng-1mRNA by RT-PCR quantitative analysis was higher than that in non-targeted microbubbles delivery group(1.04±0.08 vs 0.53±0.04,P0.01).Conclusion Microbubble carrying anti-ICAM-1 and hAng-1 gene formed a new type of targeted microbubble,it can significantly improve the transfection efficacy of hAng-1 gene expression in vivo,and it also can improve the partial angiogenesis effects after myocardial infarction.