Construction of nude mouse model of bladder carcinoma in situ and effect of VEGFsiRNA transfection on tumor

Objective To establish the nude mouse model of human bladder carcinoma in situ and observe the formation course of human bladder transitional cell carcinoma in mouse bladders,which induced by VEGFsiRNA-transfected T24 cells or untransfected T24 cells.Methods The nude mice were transplanted with VEGFsiRNA-transfected T24 cells or untransfected T24 cells into their bladders.The imageologic alterations were observed periodicly with MRI examination and the mice were sacrificed 48 d later.The weight and the volume of the tumor were tested,and the histopathologic and cytologic test were performed at the same time.Results The total ratio of the tumor formation as 90 percent(36/40)and the tumor growth speed in transfected group was much slower than that in untransfected group.The tumor weights in transfected group and untransfected group were(1.1±0.3)g and(1.6±0.5)g,respectively,and the volumes of the tumor in transfected and untransfected groups were(805±172)mm3 and(1389±294)mm3.The differences of tumor weight and volume between two groups were both significant(P0.05).While the cell grade and malignant degree in untransfected group were higher than those in transfected group.Conclusion The nude mouse model of human bladder carcinoma in situ can simulate the character of intra-bladder lacuna-growing of human bladder carcinoma in situ.The nude mouse model of human bladder carcinoma in situ has great importance in the research and exploration of new intro-bladder antineoplastic biological agent.It is an important way to value the effectiveness of chemical anticancer drugs preclinically.