Partners cells and their screening method for manufacturing a rat monoclonal antibody

Disclosed are those which can readily manufactured by a simple method, high fusion efficiency with rat lymphocytes, and have no despite vigorous proliferation potency in cancer cells, stable an object of the present invention is to provide a rat monoclonal antibody produced for the partner cells. The present invention also aims to provide a simple manufacturing method of the rat monoclonal antibody fabrication partner cell, a method for producing a monoclonal antibody using the rat monoclonal antibody fabrication partner cells. A rat monoclonal antibody fabrication partner cells according to the present invention are those derived from rat lymphocytes, have been expressed TERT gene and CyclinD1 gene, and, with respect to rat myeloma cell line YB2 / 0, expression of c-Myc gene, while 60% or less, and wherein the expression level of p16 / p19 gene is 300% or more. .BACKGROUND