Antioxidant Effect of Phenolic Fractions from Scrophularin ningpoensis Hemsl

Dried roots from Scrophularia ningpoensis Hemsl. Were extracted three times (solid: solvent = 1:7) with ethyl acetate, hexane and water to speparate phenolic compounds. e-carotene--linoleate bleaching, 1, 1-diphenyl-2-picrylhydrazyl (DPH) radical scavenging activities and activity of inhibition of corn oil oxidation were determined to evaluate the anti-oxidant activities of all extracts. The results showed that three phenolic ingredients were determined and identified from extracts. The ethyl acetate extracts gave highest content of phenolic compounds. And methanol and ethyl acetate extracts gave the highest DPH activity. All extracts greatly inhibited the oxidation of e-carotene under experiment conditions. The addition of PRFs reduced the contents of oil peroxide anisidine. syringic, hydroxybenzoic and p-cumaric acids were identified and quantified as the main phenolic ingredients of extracts.