Influence of carbon dioxide on the impregnation of the nervous tissue by silver

It has been shown that 4% carbon dioxide (CO2) in the air above reaction mixture inhibits the initiation of the formation of silver nanoparticles from complexes with biogenic amines (noradrenaline and serotonin). At the same concentration of CO2 in the air above solution of AgNO3, which is used for staining nerve tissues by the method of Golgi, neurons are preferentially stained, whereas at a concentration of 0.06%, vessels are stained. It is suggested that the entry of free silver ions to neurons is due to the inhibition of sites of initiation of silver nanoparticles in vessels at high CO2 concentrations, while the lack of inhibition leads to silver precipitation in vessels at low CO2 concentrations. It can be assumed that, for stable silver impregnation, the concentration of CO2 must be controlled.