Seeding method of target genome DNA fragment-containing plants

2014 
The invention provides a method for rapidly and accurately seeding target genome DNA fragment-containing plants on the basis of a whole-genome seeding technology. The method comprises the following steps: 1, hybridizing, backcrossing and selfing a donor plant parent containing the fragment and a recurrent parent which is a receptor plant parent free from the target genome DNA fragment; 2, using a foreground selection marker in the breeding process to carry out foreground selection; 3, using a high density molecular marker detection technology in the breeding process to carry out whole genome background selection; and 4, using above steps until the target plants with homologous recombination of two sides of the target genome DNA fragment, target genome DNA fragment homozygosis and complete background return. The invention also provides a seeding method of blast-resistant gene recombinant DNA fragment-containing plants. The stable plants having background being completely consistent with that of the receptor parent and only containing the donor parent target genome DNA fragment can be rapidly obtained through accurate foreground selection and high density molecular marker whole genome background selection.
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