A New Overview on the Old Topic: The Theoretical Analysis of "Combinatorial Strategy" for DNA Recombination

To clone genes of interest into suitable vectors is the first step to investigate their functions in vitro and in vivo . At the genome era, the sequences of more and more genes were decoded and available gradually. Therefore, it is critical to develop high efficient strategies for cloning genes of interest into different vectors to facilitate the functional analyses of them. In our previous studies, we created “Combinatorial strategy” for DNA recombination. Here, I theoretically analyzed the procedure of DNA recombination, the mechanism of this strategy, and further gave suggestions and predictions for various ligation-dependent molecular cloning experiments.