Simultaneous lipid and content mixing assays for in vitro reconstitution studies of synaptic vesicle fusion

This protocol monitors how fusion of proteoliposomes containing the vesicle-associated SNARE synaptobrevin with proteoliposomes containing the target-associated SNAREs syntaxin-1 and SNAP-25 depends on Munc18-1, Munc13-1, synaptotagmin-1, NSF and α-SNAP.