Autologous bioscaffolds based on different concentrations of platelet rich plasma and synovial fluid as a vehicle for mesenchymal stem cells

In the field of tissue engineering, diverse types of bioscaffolds are being developed currently for osteochondral defect applications. In this work, a novel scaffold based on Platelet Rich Plasma and hyaluronic acid with Mesenchymal stem cells (MSCs) has been evaluated to observe its effect on immobilized cells. The bio-scaffolds were prepared by mixing different volumes of synovial fluid (SF) with Platelet Rich Plasma (PRP) from patients obtaining 3 formulations at PRP-SF ratios of 3:1, 1:1 and 1:3 (vol/vol). The live/dead staining revealed that although the cell number of each type of bioscaffold was different, this constructs provide cells with a suitable environment for their viability and proliferation. Moreover, immobilized MSCs showed their ability to secrete fibrinolytic enzymes, which vary depending on the fibrin amount of the scaffold. Immunohistochemical analysis revealed the positive staining for collagen type II in all cases, proving the biologic action of synovial fluid derived MSCs together with the suitable characteristics of the bioscaffold for chondrogenic differentiation. Considering all these aspects, this study demonstrates that these cells-based constructs respresent an attractive method for cell immobilization, achieving completely autologous and biocompatible scaffolds. This article is protected by copyright. All rights reserved.