Effect of Thidiazuron on Somatic Embryogenesis of African Violets Leaf Explants

Effect of TDZ on formation and types of calli,induction and development of somatic embryos on leaf explants of 2 African violets varieties,Elizabeth and Nilson Blue were studied.The result showed that there were significant differences on response of culture regeneration potential and approach of morphogenesis between Elizabeth and Nilson Blue.TDZ increased the rate of callus formation and promoted the growth of calli with a suitable range of 0.5-1.0 mg·L-1.By combining with NAA,TDZ induced embryogenic callus formation and somatic embryogenesis actively.Scanning electron microscopy(SEM) observation indicated that TDZ prompted the development of somatic embryos with increasing of TDZ concentration,thus improved synchronization level of somatic embryos.MS + 0.2 mg·L-1 NAA + 1.0 mg·L-1 TDZ was the best somatic embryo induction medium for leaf explants of Elizabeth.