Study of the expression profile of immunogenesis associated genes in tuberculosis by microarray

Objective To explore the difference of gene expression profile in tuberculosis patients. Methods mRNA levels of pleural fluid and peripheral blood mononuclear cells(PBMC) in tuberculous pleurisy and lung cancer patients were compared by cDNA microarray. Paired mRNAs from fluid specimens of tuberculosis and lung cancer cases were labeled with different fluorochromes during cDNA probe synthesis in a reverse-transcription reaction. The signal intensity of each spot was measured by laser scanner and gene expression was quantified as the tubercle-to-normal fluorescence ratio (T∶N ratio). The gene was defined as over expression when the T∶N ratio was greater than 2.0 and under expression when the ratio was less than 0.5. Results Among 626 immunogenesis associated genes there were 53 differences, of which 31 (tnf-α,iglλ,il-17, il-17r, hla-dp, lcp1, tcrα, tcrβ, hsp75, cxcr4, fyb,hla-g, hla-a, il18bp, il-2r, lt-β, il-8, ip-10, mcp-1, il-12, il-12r, il-10, canx, irf2, ifn-γ, tlr, il-1, il-7, tlr, lsp-1, il-14)were higher and 22 (il-4, il-18, il-15, ifg-1, scya14, ablim, peci, ppid, hsf 2, actg2, maoa, ttid, gatm, tgfb3, insr, thbd,trap1, tcrγ, tcrδ, il-13r, il-11, igf1, a2m)were lower in tuberculous pleurisy than those in the control. Conclusions The immunogenesis of tuberculosis involves multi-genetic expression changes, such as tnf-α, il-17, il-12, tcrα,tcrβ,hsp75,cxcr4,il-4,il-18,il-15 etc., the expression profile of which changed dramatically. The results provide new insight for understanding of the pathogenic mechanisms of tuberculosis and exploring new therapeutic strategies.