Expressions of cytoprotective proteins for human renal allografts

Objective To investigate the expression status and clinical significance of cytoprotective proteins to human renal allografts.Methods Expressions of cytoprotective proteins: antiapoptotic protein(A20),heme-oxygenase-1(HO-1),and B-cell lymphoma/leukemia-XL(Bcl-XL) were analyzed by immunohistochemistry and compared in 30 renal allografts,including 10 grafts undergoing acute rejection(AR),10 grafts undergoing chronic rejection(CR),and 10 nonrejecting(NR) grafts.Results Expressions of A20,HO-1 and Bcl-XL localized mainly in endothelial,smooth muscle,and infiltrating mononuclear cells.A20 expressed in grafts undergoing AR(5.04±0.71) and CR(3.20±0.64),not in NR grafts,and its expression in CR was weaker than that in AR(P0.01).HO-1 expressed in AR(7.91±2.24),not in CR and NR.Bcl-XL was detected in all grafts(AR: 10.62±3.17;CR: 8.50±2.45;NR: 11.03±2.77),but had a decreased expression levels in CR.Conclusion A20 and HO-1 may play protective role for AR,and A20 may be the essential protein having protective function for CR.