Detection of IL-4 and IFN-γ mRNA cytokine secretion from sensitized caprine antigen presenting cells induced culture by semi-quantitative RT-PCR

RT-PCR technique had been chosen to study the caprine antigen presenting cells induced Th cells cytokine secretion profile. Macrophages induced more IFN-γ than IL-4 cytokine mRNA and in contrast, B cells induced higher IL-4 production than IFN-. In general, IL-4 mRNAs were more in two antigen pulsed cultures as assessed by integrated densitometry analysis. M. paratuberculosis induced higher IFN-mRNAs than S. aureus. Dexamethasone (50μg/ml) increased the IL-4 production than indomethacin. Thus, RT-PCR based assay system for the detection of cytokine is a fast, economical method to assess simultaneously several cytokines during an immune response. But further studies are needed to develop a competitive RT-PCR assay system to quantify the cytokines secreted by caprine effector cells at various stages of infection.