Establishment of Chinese hamster ovary cell lines stably expressing the cloned human type 1 angiotensin II receptor and characterization of the expressed receptor

We have isolated the human angiotensin II (AII) type I receptor (AT 1 ) gene,whose coding region was contained within a single exon and have established Chinese hamster ovary (CHO) cell lines stably expressing this recombinant receptor. The expressed receptor revealed the typical binding characteristics of the AT 1 receptor to several AII receptor agonists and antagonists. Treatment of the intact CHO cells with the sulfhydryl reducing reagent dithiothreitol predominantly decreased the radioligand binding, indicating that a disulfhydryl bond(s) necessary for AII binding is located on extracellular domains of the receptor molecule