Effects of tetrachlorobiphenyl on the exogenous primordial germ cells in chick.

To study the effects of tetrachlorobiphenyl on the migration of the primordial germ cells (PGCs) multiplication with utilizing poultry embryonic primordial germ cell migration features,the donor PGCs isolated from the chick embryos at stage 14 were labelled with PKH26. The labelled PGCs were transplanted into the germinal crescents of recipient embryos treated with tetrachlorobiphenyl,which were then incubated. The labelled donor PGCs were detected at stages 13～15 and stages 27～29. The number of the exogenous PGCs decreased significantly at stages 13～15 and stages 27～29 compared with controls (P0.01). Tetrachlorobiphenyl had significantly effect on the migration of exogenous PGCs,but estradiol had no effect. The result suggests that tetrachlorobiphenyl may have the impact of toxicity,rather than estrogen-like role.