Transcriptome- Based Analysis of EPO Dependent Cfu-e to Erythroblast Development

Abstract 3188 To provide molecular insight into erythroid developmental programs, including EPO- regulated aspects, we have employed transcriptome-based approaches to analyze the stage-wise development of purified murine bone marrow- derived CFUe, proerythroblasts and maturing erythroblasts. In vivo and ex vivo, these progenitors develop as Kit pos CD71 high Ter119 neg ; Kit neg CD71 high Ter119 neg ; and Kit neg CD71 high Ter119 pos cohorts (designated as E1, E2, E3 stages, respectively). In the context of EPO- modulation, stage E1 cells exhibited ∼250 EPO- regulated target genes. In stage-E2 proerythroblasts, in contrast, 750 transcripts proved to be EPO- regulated while stage-E3 erythroblasts exhibited only select EPO- modulated genes ( Disclosures: No relevant conflicts of interest to declare.